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K-12043-D20 WesternBright Sirius Kit for 2000 cm2 Membrane
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K-12043-D20 WesternBright Sirius Kit for 2000 cm2 Membrane
WesternBright Sirius is the most sensitive chemiluminescent substrate available for Western blotting.
K-12043-D20 WesternBright Sirius Kit for 2000 cm2 Membrane
Item #: 221532092

K-12043-D20 WesternBright Sirius Kit for 2000 cm2 Membrane

Item #: 221532092

BWP 13637

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WesternBright Sirius is the most sensitive chemiluminescent substrate available for Western blotting.
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What Stands Out

High Membrane Coverage
Designed for 2000 cm2 membranes, ensuring comprehensive and efficient coverage for various applications, optimizing performance across different environments.
Advanced Brightness
Equipped with WesternBright technology, this kit offers superior signal clarity and brightness, enhancing visualization for improved analysis in scientific research.
User-Friendly Design
This kit includes easy-to-follow instructions, making it accessible for both novice and experienced users, reducing setup time and improving overall workflow efficiency.

Product Details

Shop K-12043-D20 WesternBright Sirius Kit for 2000 cm2 Membrane online at a best price in Botswana. B01MT3TXYL

Who Should Buy?

Suitable For
  • Life Science Researchers

    Ideal for researchers needing high-quality detection of proteins in western blotting for valid and reproducible results.

  • Laboratory Technicians

    Perfect for technicians performing various gel blotting assays requiring a reliable detection system for protein analysis.

  • Educational Institutions

    Beneficial for educational labs focusing on molecular biology experiments, teaching students efficient protein analysis techniques.

Not Suitable For
  • Budget-Conscious Users

    Less suitable for users looking for a low-cost option as this kit may be priced higher than alternatives.

Product Description

K-12043-D20 WesternBright Sirius Kit for 2000 cm2 Membrane

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Customer Questions & Answers

  • Question: What is the K-12043-D20 WesternBright Sirius Kit designed for?

    Answer: The K-12043-D20 WesternBright Sirius Kit is designed for effective protein detection and analysis in scientific research. This kit utilizes advanced technology to visualize and quantify proteins on membranes, making it ideal for applications in molecular biology, biochemistry, and related fields. By allowing researchers to assess protein expression levels accurately, it is beneficial in studies involving gene function, cellular mechanisms, and disease research.
  • Question: How do I use the K-12043-D20 WesternBright Sirius Kit?

    Answer: Using the K-12043-D20 WesternBright Sirius Kit involves preparing your membrane with the desired sample, followed by blocking to prevent non-specific binding. Afterward, you incubate the membrane with primary antibodies, followed by secondary antibodies conjugated to a detection enzyme or fluorophore. Finally, the kit provides necessary reagents for visualization. This systematic approach ensures sensitivity and specificity in protein detection, making it suitable for various laboratory applications.
  • Question: What types of membranes can I use with the K-12043-D20 kit?

    Answer: The K-12043-D20 WesternBright Sirius Kit is compatible with a range of membranes including PVDF (polyvinylidene fluoride) and nitrocellulose. These membranes effectively support protein binding during the western blotting process. Such versatility allows researchers to choose the ideal membrane type suited for their specific experimental needs, ensuring optimal results for both qualitative and quantitative analysis.
  • Question: How sensitive is the K-12043-D20 WesternBright Sirius Kit?

    Answer: The K-12043-D20 WesternBright Sirius Kit is highly sensitive, capable of detecting low nanogram levels of proteins. This sensitivity is crucial for studies that involve low-abundance proteins or when working with small sample sizes. Enhanced detection capabilities empower researchers to uncover more subtle biological signals in their experiments, thus facilitating deeper insights into cellular function and pathology.
  • Question: Can I multiplex with the WesternBright Sirius Kit?

    Answer: Yes, the K-12043-D20 WesternBright Sirius Kit facilitates multiplexing, allowing you to detect multiple proteins in a single blot. By utilizing different primary antibodies that are compatible in terms of species and detection methods, researchers can gain comprehensive data from one experiment. This capability is particularly efficient for comparative studies where relative protein expression of multiple targets is required.
  • Question: What detection methods can I use with this kit?

    Answer: The K-12043-D20 WesternBright Sirius Kit supports various detection methods, including chemiluminescence and fluorescence. Depending on your research needs, chemiluminescence detection offers high sensitivity, while fluorescence allows for simultaneous multiplexing. These methods provide flexibility in data analysis and visualization, enabling researchers to choose the best approach for their specific experimental objectives.
  • Question: Does the K-12043-D20 kit require special equipment?

    Answer: While the K-12043-D20 WesternBright Sirius Kit does not require specialized equipment, a basic western blotting setup, including a gel electrophoresis apparatus, blotting unit, and imaging system (chemiluminescent or fluorescence) is essential. These tools are common in most molecular biology labs. This accessibility aids researchers in smoothly integrating the kit into their existing workflows without needing substantial additional investment.
  • Question: What are common applications for the K-12043-D20 WesternBright Sirius Kit?

    Answer: The K-12043-D20 WesternBright Sirius Kit is commonly used for applications such as protein expression analysis, post-translational modifications assessment, and verification of gene silencing effects. It is suitable for diverse research areas, including cancer biology, immunology, and pharmacology. By providing reliable results in these applications, the kit serves as a foundational tool for academic research and pharmaceutical development.
  • Question: Can I optimize my results with this kit?

    Answer: Yes, optimizing results with the K-12043-D20 WesternBright Sirius Kit is possible through careful consideration of several factors, including antibody concentrations, incubation times, and temperature settings. Tailoring these parameters based on specific experimental conditions enhances sensitivity and reduces background noise. Such optimizations enable researchers to achieve clearer, more reliable results, increasing the overall quality of their work.
  • Question: Where can I buy K-12043-D20 WesternBright Sirius Kit for 2000 cm2 Membrane?

    Answer: You can purchase the K-12043-D20 WesternBright Sirius Kit for 2000 cm2 Membrane on Ubuy, a reliable e-commerce platform that provides a wide range of scientific products. Ubuy is known for its extensive selection, competitive prices, and convenient shopping experience, ensuring you have full access to browse and obtain this essential kit for your research needs.

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Features & Benefits

  • Most sensitive substrate for Western blotting.
  • Extremely strong signal enhances visibility of bands.
  • Detects bands that are invisible with other substrates.
  • Offers quantitative signal for accurate measurements.
  • Emits chemifluorescence for use with fluorescence imagers.
  • Ideal for high-quality and reliable results.

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