CryoKing
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HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
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HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
Stable Buffering Capacity: 1M HEPES buffer with a stable pH of 7.3 ± 0.1, ideal for preparing cell culture buffers and other laboratory applications requiring precise pH control.
HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case
Item #: 255802040

HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6

Item #: 255802040

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Stable Buffering Capacity: 1M HEPES buffer with a stable pH of 7.3 ± 0.1, ideal for preparing cell culture buffers and other laboratory applications requiring precise pH control.
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Shop HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Prepare Protein Extraction Buffer, Cell Culture Buffer, Ready to Use Solution, 100ml/Bottle, 6 Bottles/Case online at a best price in Botswana. B0FG2H9S1Y
Brand NameCryoKing
Unit Count6 Count
ManufacturerBIOLOGIX
Included ComponentsHEPES Buffer
Part NumberHEPES-100-6
BrandCryoKing
Item Weight0.55 lbs (250 grams)

What's in the box

HEPES Buffer

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Customer Questions & Answers

  • Question: What is HEPES Buffer and what is it used for?

    Answer: HEPES Buffer is a zwitterionic buffering agent commonly used in biological and biochemical applications. It maintains a stable pH, making it ideal for preparing protein extraction buffers and cell culture media. HEPES is particularly effective in maintaining optimal pH in environments sensitive to changes, such as live cell cultures, where consistent pH is crucial for cell viability and function.
  • Question: How is the HEPES Buffer prepared?

    Answer: The HEPES Buffer is formulated and pre-mixed for your convenience, ensuring accurate pH levels of 7.3. For laboratory use, simply dilute the buffer as required for your specific application, such as protein extraction or cell culture. The pre-sterilized formulation minimizes contamination risks, allowing for precise and reliable experimental results.
  • Question: Why is a pH of 7.3 ideal for cellular applications?

    Answer: A pH of 7.3 closely resembles the physiological conditions in mammalian cells, making it optimal for in vitro experiments. Maintaining this pH level helps ensure that cellular processes, such as enzyme activity and protein interactions, proceed normally. This characteristic is particularly beneficial during protein studies or culturing cells, as it supports cell health and experimental accuracy.
  • Question: What advantages does the 0.1 um filtration provide?

    Answer: The 0.1 um filtration ensures that the HEPES Buffer is sterile and free from contaminants, including bacteria and larger particles that might compromise your experiments. This level of filtration is critical when working with sensitive biological samples, where contamination could drastically alter results. Using pre-filtered buffers helps uphold the integrity of your research.
  • Question: In what types of experiments can HEPES Buffer be used?

    Answer: HEPES Buffer is versatile and can be used in various applications, including protein extraction, enzymatic assays, and cell culture systems. It's particularly effective in scenarios that involve delicate cellular processes or reactions sensitive to pH fluctuations, enhancing the accuracy and reliability of results across different research fields.
  • Question: Are there any storage considerations for HEPES Buffer?

    Answer: To maintain the effectiveness of HEPES Buffer, it should be stored in a cool, dry place, away from direct sunlight. After opening, it is best to use the buffer within a reasonable timeframe to ensure its stability and performance. Proper storage helps prevent degradation of the buffer components, keeping it ready for immediate use in your experiments.
  • Question: Can HEPES Buffer be used in combination with other reagents?

    Answer: Yes, HEPES Buffer can be safely mixed with various biological reagents in many applications, including other buffers or salts used in cell culture and protein assays. It's important to test compatibility with specific reagents in your experimental protocol to ensure that the buffer does not interfere with their function. This adaptability makes HEPES a go-to choice in many research settings.
  • Question: Is HEPES Buffer suitable for long-term cell culture?

    Answer: HEPES Buffer is suitable for use in long-term cell culture due to its ability to stabilize pH over extended periods. However, the buffer should be refreshed periodically to keep optimal conditions. Utilizing HEPES in cell culture experiments can help ensure cells remain healthy and proliferate effectively, thus contributing to successful experimental outcomes.
  • Question: How does HEPES Buffer compare to other buffers?

    Answer: HEPES Buffer is favored over other buffers like PBS or Tris in specific applications where pH stability is crucial in the presence of biological materials. Its low UV absorbance and effective buffering capacity at physiological pH make it particularly useful for live cell studies, where other buffers may not perform as well under similar conditions.
  • Question: Where can I buy HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH?

    Answer: You can buy HEPES Buffer, 0.1um Filtration Sterilized, 7.3 pH, for Preparing Protein Extraction Buffer and Cell Culture Buffer from Ubuy. They offer this ready-to-use solution in a convenient 100ml bottle, making it accessible for all your laboratory needs in Botswana.

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Features & Benefits

  • Stable pH of 7.3 ± 0.1 for precise buffering.
  • Minimized endotoxin levels ensure high purity.
  • Long shelf life of at least 3 years when stored properly.
  • Batch consistency ensures reliable performance.
  • Suitable for various laboratory applications.
  • Ideal for sensitive experiments requiring stability.

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